Primeiro relato de Trichoderma em Pergamino, Buenos Aires, Argentina
DOI:
https://doi.org/10.22579/20112629.861Keywords:
biological control, Internal Transcribed Spacers (ITS), phytopathogens, HypocreaceaeAbstract
Introduction. Trichoderma’s biological capacity is very important as a potential biological control agent. They are among the most studied soil fungi and are commercially available as fungicides. Objective. To identify and molecularly characterize an isolate of Trichoderma sp. Materials and methods. Mycelium was isolated from soybean roots at the Phytopathology Laboratory of the National University of the Northwest of the Province of Buenos Aires, Pergamino, Argentina,in 2023. The isolate was analyzed macroscopically. The sequence of Internal Transcribed Spacers (ITS) was amplified by polymerase chain reaction (PCR) using the ITS1 and ITS4 primers and sent for sequencing to Macrogen (Korea). Results. Macroscopically, the isolate was green. The amplified ITS sequence showed 99.13% identity with the MH284962 sequence of Trichoderma sp. Conclusion. A Trichoderma sp. isolate was successfully identified and molecularly characterized.
Downloads
References
Alwadai, A. S., Al Wahibi, M.S., Alsayed, M.F. (2024). Caracterización molecular de Trichoderma promotora del crecimiento vegetal de Arabia Saudita. Sci Rep, 14, 23236. https://doi.org/10.1038/s41598-024-73762-5
Pavone, D. (2012). Biocontrol de Rhizoctonia solani Kühn por Trichoderma spp. [Tesis Doctoral en Ciencias Mención Biología Celular, Universidad Central de Venezuela]. 192 p.
Leelavathi, M. S, Vani, L., & Reena, P. (2014). Antimicrobial activity of Trichoderma harzianum against bacteria and fungi. International Journal of Current Microbiology and Applied Sciences, 3(1), 96-103. http://www.ijcmas.com/vol-31/M.S.Leelavathi,%20et%20al.pdf
Kumar Solanki, M., Singh, N., Kumar Singh, R., Singh, P., Srivastava, A. K., Kumar, S., Arora, D. K. (2011). Plant defense activation and management of tomato root rot by a chitin-fortified Trichoderma/Hypocrea formulation. Phytoparasitica, 39, 471-481.doi:10.1007/s12600-011-0188-y
White, T., Bruns, T., Lee, S., & Taylor, J. (1990). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. PCR Protocols. Academic Press. https://doi.org/10.1016/b978-0-12-372180-8.50042-1
Hernández, M. A., Muiño, B. L., Roson, A. C. & Cazola, G. C. (2011). Hongos y oomycetes fitopatógenos en viveros de piña Ananas comosus (L.) merril en Ciego de Ávila, Cuba. Fitosanidad, 15(3), 137-142.
Cañedo, V., & Ames, T. (2004). Manual de laboratorio para el manejo de hongos entomopatógenos. Centro Internacional de la Papa (CIP),62 p. Lima, Perú.
Hermosa, M. R., Grondona, I., Iturriaga, E. A., Diaz-Minguez, J. M., Castro, C., Monte, E., & Garcia-Acha, I. (2000). Molecular Characterization and Identification of Biocontrol Isolates of Trichoderma spp. Applied and Environmental Microbiology, 66(5), 1890-1898. https://doi.org/10.1128/aem.66.5.1890-1898.2000
Druzhinina, I. S., Kopchinskiy, A. G. and Kubicek, C.P. (2006). The first 100 Trichoderma species characterized by molecular data. Mycoscience, 47, 55-64.
García-Núñez, H. G., Martínez-Campos, Á. R., Hermosa-Prieto, M. R., Monte-Vázquez, E., Aguilar-Ortigoza, C. J., & González-Esquivel, C. E. (2017). Caracterización morfológica y molecular de cepas nativas de Trichoderma y su potencial de biocontrol sobre Phytophthora infestans. Revista Mexicana de Fitopatología, 35(1), 58-79. https://doi.org/10.18781/r.mex.fit.1605-4
Matas-Baca, M. A., Urías García C., Pérez-Álvarez, S., Flores-Córdova, M. A., Escobedo-Bonilla, C. M., Magallanes-Tapia, M. A., Sánchez Chávez, E. (2022). Morphological and molecular characterization of a new autochthonous Trichoderma sp. isolate and its biocontrol efficacy against Alternaria sp. Saudi Journal of Biological Sciences, 29(4). https://doi.org/10.1016/j.sjbs.2021.12.052.
Downloads
Published
License
Copyright (c) 2026 Orinoquia
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
